Race: Human

Birthday: March 14

Birthplace: 4th Hierarchical City of Naobi

Height: 160 cm

Weight: 47 kg

Blood type: A

Hobbies: Singing

Likes: Historical dramas

Dislikes: War

During the Military Academy, she was close friends with Noel and Makoto. She's described as a straight A student, and has a rather no-nonsense personality. She's very serious and reserved, and is a member of the dignified Yayoi family. By the end of BlazBlue: Calamity Trigger's True Ending, she is assigned by Hazama to kill Noel and Jin. She originally appeared as a non-playable character in the Calamity Trigger Story Mode. In BlazBlue: Continuum Shift, Tsubaki heads to 13th Hierarchical City of Kagutsuchi to search for Jin Kisaragi and Noel Vermillion.

She uses an Ars Armagus called "Izayoi". When Tsubaki found Jin, he told her to get rid of Izayoi because it "steals the light from the user's eyes", implying it would, after a certain period of time, cause her to go blind.

Tsubaki is revealed to be a fan of Hakumen, going so far as to own all the books and movies based on the exploits of the Six Heroes.